Physiological response, testicular function, and health indices of rabbit males fed diets containing phytochemicals extract under heat stress conditions.

OBJECTIVE: The present study aimed to evaluate the impact of dietary turmeric or garlic extract on physiological responses, hematological parameters, antioxidants status, testicular functions, and health indices of heat-stressed rabbit males. MATERIALS AND METHODS: Seventy bucks were distributed into seven experimental groups (ten/group). A commercial diet (CD) was fed to rabbit males in the control group, while males in G2, G3, and G4 were fed CD with turmeric at 30, 60, and 90 mg/kg, respectively. Males in G5, G6, and G7 were fed CD with garlic extract at 50, 75, and 100 mg/kg, respectively, for 8 weeks. RESULTS: The results showed that turmeric or garlic extract levels decreased ear temperature, respiration rate, germ cell apoptotic number, total cholesterol, triglycerides, malondialdehyde level, libido, and sperm of dead and abnormal tail (p < 0.05). The hemoglobin and red and white blood cells, platelets, total antioxidants capacity, mass motility, mitochondrial potential, normal, acrosome reacted, normality, and a total functional fraction of spermatozoa and relative of epididymal weight were increased (p < 0.05). The increased packed cell volume percentage and initial semen fructose were significant only when 100 mg per kg garlic extract was supplemented. CONCLUSION: Phytochemicals extract supplementation can be an effective dietary supplementation to eliminate heat stress and improve health, oxidative capacity, and testicular functions of rabbit males.

Relief of the negative effects of heat stress on semen quality, reproductive efficiency and oxidative capacity of rabbit bucks using different natural antioxidants.

OBJECTIVE: The potential of extra virgin olive oil (EVOO), betaine (BET), and ginger (GIN), as natural antioxidants, in reducing negative effects of heat stress on physiological responses, antioxidant capacity, semen quality and fertility of bucks under heat stress were investigated. METHODS: Forty adult Animal Production Research Institute line rabbit bucks were distributed randomly into four experimental treatments of ten rabbits each. The first treatment was fed the commercial pellet diet (CPD) without supplementation and served as a control. The other three treatments were fed CPD supplemented with EVOO (300 mg), BET (1,000 mg), and GIN (200 mg) per kg diet for 3 consecutive months during the summer season. RESULTS: Supplementation of EVOO, BET, or GIN improved (p<0.05) the sexual desire, progressive motility, vitality, intact acrosome and membrane integrity, sperm cell concentration, sperm outputs and fertility. Seminal plasma total proteins, globulin, total antioxidant capacity, glutathione and glutathione S-transferase, and initial fructose increased (p<0.05), while total lipids, aspartate and alanine aminotransferases and malondialdehyde decreased (p<0.05) compared with the control. In comparing the natural antioxidants treatments, GIN evoked the largest improvement. CONCLUSION: The inclusion of GIN (200 mg/kg diet) appeared to improve the sexual desire, semen quality and oxidative stress of bucks. This may be a beneficial supplement for the management of rabbit bucks used in natural mating or artificial insemination.

Effect of Spirulina and Vitamin E on Reproduction and in vitro Embryo Production in Heat-stressed Rabbits.

BACKGROUND AND OBJECTIVE: High ambient temperature can cause heat stress and evokes a combination of change in blood biochemicals and reproduction of rabbit. This study targeted to investigate the effect of Spirulina platensis, vitamin E and their combination on in vivo and in vitro reproductive performance and some physiological and health indicators of heat stressed rabbit does. MATERIALS AND METHODS: Nili-parous rabbit does (n = 80) were allocated to 4 groups. Does in the 1st group were fed commercial complete feed diet, while those in the 2nd, 3rd and 4th were fed complete feed diet with Spirulina platensis (300 mg kg-1), vitamin E (100 mg kg-1 diet) and Spirulina platensis+vitamin E kg-1 diet, respectively. All does were naturally mated with fertile bucks (5 bucks/group). RESULTS: The does in the 2nd group showed significantly (p<0.05) better reproductive performance (conception rate, kindling rate and litter size), lipid profile (total lipids, cholesterol, triglycerides, high and low density lipoproteins, antioxidant capacity (total antioxidant capacity, glutathione, malondialdehyde, glutathione peroxidase, glutathione S-transferase, superoxide dismutase and catalase), immunity (lysozyme, IgG and IgM), ovulatory response (corpora lutea number and ovulation rate), embryo quality and hatched blastocysts production with higher cell number and inner cell mass as compared to other groups. CONCLUSION: Dietary supplementation with Spirulina platensis (300 mg kg-1 diet), in comparing with vitamin E (100 mg kg-1 diet) or their combination at the same levels, had positive impact on reproductive performance of rabbit does used in breeding program under heat stress condition in Egypt.